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Minimizing Back Exchange in (18)O(/16)O Quantitative Proteomics Experiments by Incorporation of Immobilized Trypsin into the Initial Digestion Step

Differential labeling of peptides via the use of the (18)O water proteolytic labeling method has been widely adopted for quantitative shotgun proteomics studies due to its simplicity and low reagent costs. In this report, the use of immobilized trypsin in the initial digestion step, in addition to t...

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Detalhes bibliográficos
Main Authors: Sevinsky, Joel R., Brown, Kristy J., Cargile, Benjamin J., Bundy, Jonathan L., Stephenson, James L.
Formato: Artigo
Idioma:Inglês
Publicado em: 2007
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2796076/
https://ncbi.nlm.nih.gov/pubmed/17249691
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ac0620819
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