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Minimizing Back Exchange in (18)O(/16)O Quantitative Proteomics Experiments by Incorporation of Immobilized Trypsin into the Initial Digestion Step
Differential labeling of peptides via the use of the (18)O water proteolytic labeling method has been widely adopted for quantitative shotgun proteomics studies due to its simplicity and low reagent costs. In this report, the use of immobilized trypsin in the initial digestion step, in addition to t...
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| Autors principals: | , , , , |
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| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
2007
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2796076/ https://ncbi.nlm.nih.gov/pubmed/17249691 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ac0620819 |
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