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Application of pressurized solvents for ultra fast trypsin hydrolysis in proteomics: Proteomics on the fly

A new method for rapid proteolytic digestion of proteins under high pressure that uses pressure cycling technology in the range of 5 to 35 kpsi was demonstrated for proteomic analysis. Successful in-solution digestions of single proteins and complex protein mixtures were achieved in 60 s and then an...

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Autors principals: López-Ferrer, Daniel, Petritis, Konstantinos, Hixson, Kim K., Heibeck, Tyler H., Moore, Ronald J., Belov, Mikhail E., Camp, David G., Smith, Richard D.
Format: Artigo
Idioma:Inglês
Publicat: 2008
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC2744211/
https://ncbi.nlm.nih.gov/pubmed/18605748
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/pr7008077
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