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Distinct Roles of the Active-site Mg(2+) Ligands, Asp(882) and Asp(705), of DNA Polymerase I (Klenow Fragment) during the Prechemistry Conformational Transitions
DNA polymerases catalyze the incorporation of deoxynucleoside triphosphates into a growing DNA chain using a pair of Mg(2+) ions, coordinated at the active site by two invariant aspartates, whose removal by mutation typically reduces the polymerase activity to barely detectable levels. Using two sto...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
American Society for Biochemistry and Molecular Biology
2011
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3030377/ https://ncbi.nlm.nih.gov/pubmed/21084297 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M110.167593 |
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