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High affinity immobilization of proteins using biotin- and GST-based coupling strategies

Surface Plasmon Resonance (SPR) is a highly sensitive method for the detection of molecular interactions. One interacting partner is immobilized on the sensor chip surface while the other is injected across the sensor surface. This chapter focuses on high affinity immobilization of protein substrate...

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Bibliografiska uppgifter
Huvudupphovsmän: Hutsell, Stephanie Q., Kimple, Randall J., Siderovski, David P., Willard, Francis S., Kimple, Adam J.
Materialtyp: Artigo
Språk:Inglês
Publicerad: 2010
Ämnen:
Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC3025018/
https://ncbi.nlm.nih.gov/pubmed/20217614
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-60761-670-2_4
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