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A capture coupling method for the covalent immobilization of hexahistidine tagged proteins for surface plasmon resonance
Surface Plasmon Resonance (SPR) is a robust method to detect and quantify macromolecular interactions; however, to measure binding interactions, one component must be immobilized on a sensor surface. This is typically achieved using covalent immobilization via free amines or thiols, or noncovalent i...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2010
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3031178/ https://ncbi.nlm.nih.gov/pubmed/20217615 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-60761-670-2_5 |
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