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A capture coupling method for the covalent immobilization of hexahistidine tagged proteins for surface plasmon resonance

Surface Plasmon Resonance (SPR) is a robust method to detect and quantify macromolecular interactions; however, to measure binding interactions, one component must be immobilized on a sensor surface. This is typically achieved using covalent immobilization via free amines or thiols, or noncovalent i...

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Detaylı Bibliyografya
Asıl Yazarlar: Kimple, Adam J., Muller, Robin E., Siderovski, David P., Willard, Francis S.
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: 2010
Konular:
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC3031178/
https://ncbi.nlm.nih.gov/pubmed/20217615
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-60761-670-2_5
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