Surface Plasmon Resonance Imaging-Based Protein Array Chip System for Monitoring a Hexahistidine-Tagged Protein during Expression and Purification

A surface plasmon resonance imaging-based Ni(2+)-iminodiacetic acid-coated gold chip system was developed to enable specific detection of a hexahistidine-tagged recombinant protein in crude extracts or in column chromatography fractions. This system is especially advantageous for high-throughput ana...

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Detalhes bibliográficos
Main Authors: Ro, Hyeon-Su, Jung, Sun Ok, Kho, Byung Hoon, Hong, Hyung Pyo, Lee, Jae Sung, Shin, Yong-Beom, Kim, Min Gon, Chung, Bong Hyun
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2005
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Methods
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC546681/
https://ncbi.nlm.nih.gov/pubmed/15691971
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.71.2.1089-1092.2005
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