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Hexahistidine-Tag-Specific Optical Probes for Analyses of Proteins and Their Interactions
The hexahistidine (His(6))/Nickel (II)-Nitrilotriacetic Acid (Ni(2+)-NTA) system is widely used for affinity purification of recombinant proteins. The NTA group has many other applications, including the attachment of chromophores, fluorophores, or nano-gold to His(6)-proteins. Here we explore sever...
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| Main Authors: | , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2009
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2832190/ https://ncbi.nlm.nih.gov/pubmed/20036207 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2009.12.028 |
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