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Histidine-tag-directed chromophores for tracer analyses in the analytical ultracentrifuge
Many recombinant proteins carry an oligohistidine (His(X))-tag that allows their purification by immobilized metal affinity chromatography (IMAC). This tag can be exploited for the site-specific attachment of chromophores and fluorophores, using the same metal ion–nitrilotriacetic acid (NTA) coordin...
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| Autors principals: | , , , , , , |
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| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
2010
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3090473/ https://ncbi.nlm.nih.gov/pubmed/21187151 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ymeth.2010.12.033 |
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