Histidine-tag-directed chromophores for tracer analyses in the analytical ultracentrifuge

Many recombinant proteins carry an oligohistidine (His(X))-tag that allows their purification by immobilized metal affinity chromatography (IMAC). This tag can be exploited for the site-specific attachment of chromophores and fluorophores, using the same metal ion–nitrilotriacetic acid (NTA) coordin...

Disgrifiad llawn

Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Prif Awduron: Hellman, Lance M., Zhao, Chunxia, Melikishvili, Manana, Tao, Xiaorong, Hopper, James E., Whiteheart, Sidney W., Fried, Michael G.
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: 2010
Pynciau:
Article
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC3090473/
https://ncbi.nlm.nih.gov/pubmed/21187151
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ymeth.2010.12.033
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