Hexahistidine-Tag-Specific Optical Probes for Analyses of Proteins and Their Interactions

The hexahistidine (His(6))/Nickel (II)-Nitrilotriacetic Acid (Ni(2+)-NTA) system is widely used for affinity purification of recombinant proteins. The NTA group has many other applications, including the attachment of chromophores, fluorophores, or nano-gold to His(6)-proteins. Here we explore sever...

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Main Authors: Zhao, Chunxia, Hellman, Lance M., Zhan, Xin, Bowman, Willis S., Whiteheart, Sidney W., Fried, Michael G.
Format: Artigo
Jezik:Inglês
Izdano: 2009
Teme:
Article
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC2832190/
https://ncbi.nlm.nih.gov/pubmed/20036207
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2009.12.028
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