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High affinity immobilization of proteins using biotin- and GST-based coupling strategies
Surface Plasmon Resonance (SPR) is a highly sensitive method for the detection of molecular interactions. One interacting partner is immobilized on the sensor chip surface while the other is injected across the sensor surface. This chapter focuses on high affinity immobilization of protein substrate...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2010
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3025018/ https://ncbi.nlm.nih.gov/pubmed/20217614 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-60761-670-2_4 |
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