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Separation of supercoiled from open circular forms of plasmid DNA, and biological activity detection

To establish a cost-effective purification process for the large-scale production of plasmid DNA for gene therapy and DNA vaccination, a single anion-exchange chromatography (AEC) step was employed to purify supercoiled plasmid DNA (sc pDNA) from other isoforms and Escherichia coli impurities presen...

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Bibliografiske detaljer
Main Authors: Li, Huangjin, Bo, Huaben, Wang, Jinquan, Shao, Hongwei, Huang, Shulin
Format: Artigo
Sprog:Inglês
Udgivet: Springer Netherlands 2010
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3021145/
https://ncbi.nlm.nih.gov/pubmed/21120691
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s10616-010-9322-9
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