Quantitation of supercoiled circular content in plasmid DNA solutions using a fluorescence-based method

A method for quantifying the proportion of supercoiled circular (SC) forms in DNA solutions is described. The method (SCFluo) takes advantage of the reversible denaturation property of SC forms and the high specificity of the PicoGreen fluorochrome for double-stranded (ds)DNA. Fluorescence values of...

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Detalhes bibliográficos

Publicado no:	Nucleic Acids Res
Principais autores:	Levy, M. S., Lotfian, P., O’Kennedy, R., Lo-Yim, M. Y., Shamlou, P. Ayazi
Formato:	Artigo
Idioma:	Inglês
Publicado em:	Oxford University Press 2000
Assuntos:	NAR Methods Online
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC102742/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10871380/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/28.12.e57
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