Quantitation of supercoiled circular content in plasmid DNA solutions using a fluorescence-based method
A method for quantifying the proportion of supercoiled circular (SC) forms in DNA solutions is described. The method (SCFluo) takes advantage of the reversible denaturation property of SC forms and the high specificity of the PicoGreen fluorochrome for double-stranded (ds)DNA. Fluorescence values of...
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| Опубліковано в:: | Nucleic Acids Res |
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| Автори: | , , , , |
| Формат: | Artigo |
| Мова: | Inglês |
| Опубліковано: |
Oxford University Press
2000
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| Предмети: | |
| Онлайн доступ: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC102742/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10871380/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/28.12.e57 |
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