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Combination of overlapping bacterial artificial chromosomes by a two-step recombinogenic engineering method

Recombinogenic engineering or recombineering is a powerful new method to engineer DNA without the need for restriction enzymes or ligases. We report here a general method for using recombineering to combine overlapping bacterial artificial chromosomes (BACs) to build larger, unified BACs. In order t...

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Autors principals: Zhang, Xin-Mei, Huang, Jian-Dong
Format: Artigo
Idioma:Inglês
Publicat: Oxford University Press 2003
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC169968/
https://ncbi.nlm.nih.gov/pubmed/12888533
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