Combination of overlapping bacterial artificial chromosomes by a two-step recombinogenic engineering method

Recombinogenic engineering or recombineering is a powerful new method to engineer DNA without the need for restriction enzymes or ligases. We report here a general method for using recombineering to combine overlapping bacterial artificial chromosomes (BACs) to build larger, unified BACs. In order t...

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Veröffentlicht in:	Nucleic Acids Res
Hauptverfasser:	Zhang, Xin-Mei, Huang, Jian-Dong
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	Oxford University Press 2003
Schlagworte:	NAR Methods Online
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC169968/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12888533/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/gng081
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