A capture approach for supercoiled plasmid DNA using a triplex-forming oligonucleotide

Proteins that recognize and bind specific sites in DNA are essential for regulation of numerous biological functions. Such proteins often require a negative supercoiled DNA topology to function correctly. In current research, short linear DNA is often used to study DNA–protein interactions. Although...

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Autors principals: Ruigrok, Vincent J. B., Westra, Edze R., Brouns, Stan J. J., Escudé, Christophe, Smidt, Hauke, van der Oost, John
Format: Artigo
Idioma:Inglês
Publicat: Oxford University Press 2013
Matèries:
Methods Online
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC3664820/
https://ncbi.nlm.nih.gov/pubmed/23571753
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkt239
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