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SUPERCOILED CIRCULAR DNA-PROTEIN COMPLEX IN Escherichia coli: PURIFICATION AND INDUCED CONVERSION TO AN OPEN CIRCULAR DNA FORM

The 23S twisted circular form of ColE(1) DNA has been isolated from Escherichia coli as a tightly associated DNA-protein complex with a sedimentation coefficient of approximately 24S. Treatment of this complex with pronase, trypsin, sodium dodecyl sulfate, Sarkosyl, or heat results in a conversion t...

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Main Authors: Clewell, Don B., Helinski, Donald R.
格式: Artigo
語言:Inglês
出版: 1969
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC223628/
https://ncbi.nlm.nih.gov/pubmed/4894690
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