SUPERCOILED CIRCULAR DNA-PROTEIN COMPLEX IN Escherichia coli: PURIFICATION AND INDUCED CONVERSION TO AN OPEN CIRCULAR DNA FORM

The 23S twisted circular form of ColE(1) DNA has been isolated from Escherichia coli as a tightly associated DNA-protein complex with a sedimentation coefficient of approximately 24S. Treatment of this complex with pronase, trypsin, sodium dodecyl sulfate, Sarkosyl, or heat results in a conversion t...

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Pubblicato in:	Proc Natl Acad Sci U S A
Autori principali:	Clewell, Don B., Helinski, Donald R.
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	National Academy of Sciences 1969
Soggetti:	Biological Sciences: Biochemistry
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC223628/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/4894690/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.62.4.1159
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