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Separation of supercoiled from open circular forms of plasmid DNA, and biological activity detection
To establish a cost-effective purification process for the large-scale production of plasmid DNA for gene therapy and DNA vaccination, a single anion-exchange chromatography (AEC) step was employed to purify supercoiled plasmid DNA (sc pDNA) from other isoforms and Escherichia coli impurities presen...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Springer Netherlands
2010
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3021145/ https://ncbi.nlm.nih.gov/pubmed/21120691 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s10616-010-9322-9 |
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