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Role of a GAG Hinge in the Nucleotide-induced Conformational Change Governing Nucleotide Specificity by T7 DNA Polymerase

A nucleotide-induced change in DNA polymerase structure governs the kinetics of polymerization by high fidelity DNA polymerases. Mutation of a GAG hinge (G542A/G544A) in T7 DNA polymerase resulted in a 1000-fold slower rate of conformational change, which then limited the rate of correct nucleotide...

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Sonraí Bibleagrafaíochta
Main Authors: Jin, Zhinan, Johnson, Kenneth A.
Formáid: Artigo
Teanga:Inglês
Foilsithe: American Society for Biochemistry and Molecular Biology 2011
Ábhair:
Rochtain Ar Líne:https://ncbi.nlm.nih.gov/pmc/articles/PMC3020739/
https://ncbi.nlm.nih.gov/pubmed/20978284
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M110.156737
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