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Site-specific labeling of T7 DNA polymerase with a conformationally sensitive fluorophore and its use in detecting single-nucleotide polymorphisms

Like most enzymes, DNA polymerases undergo a large conformational change on the binding of a correct nucleotide. To determine how the conformational change contributes to substrate specificity, we labeled the T7 DNA polymerase with a conformationally sensitive fluorophore at a position that provides...

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書目詳細資料
Main Authors: Tsai, Yu-Chih, Jin, Zhinan, Johnson, Kenneth A.
格式: Artigo
語言:Inglês
出版: 2008
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC3045965/
https://ncbi.nlm.nih.gov/pubmed/18834851
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2008.09.006
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