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Single-fluorophore biosensors based on conformation-sensitive GFP variants
The β-strands of GFP form a rigid barrel that protects the chromophore from external influence. Herein, we identified specific mutations in β-strand 7 that render the chromophore sensitive to interactions of GFP with another protein domain. In the process of converting the FRET-based protein kinase...
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| Main Authors: | , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Federation of American Societies for Experimental Biology
2014
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3929668/ https://ncbi.nlm.nih.gov/pubmed/24334549 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1096/fj.13-240507 |
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