Tethered Fluorophore Motion: Studying Large DNA Conformational Changes by Single-fluorophore Imaging

We have previously introduced tethered fluorophore motion (TFM), a single-molecule fluorescence technique that monitors the effective length of a biopolymer such as DNA. TFM uses the same principles as tethered particle motion (TPM) but employs a single fluorophore in place of the bead, allowing TFM...

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Detalhes bibliográficos
Main Authors: May, Peter F.J., Pinkney, Justin N.M., Zawadzki, Pawel, Evans, Geraint W., Sherratt, David J., Kapanidis, Achillefs N.
Formato: Artigo
Idioma:Inglês
Publicado em: The Biophysical Society 2014
Assuntos:
Proteins and Nucleic Acids
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4156673/
https://ncbi.nlm.nih.gov/pubmed/25185556
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2014.07.024
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