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Orientation of Cyanine Fluorophores Terminally Attached to DNA via Long, Flexible Tethers
Cyanine fluorophores are commonly used in single-molecule FRET experiments with nucleic acids. We have previously shown that indocarbocyanine fluorophores attached to the 5′-termini of DNA and RNA via three-carbon atom linkers stack on the ends of the helix, orienting their transition moments. We no...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
The Biophysical Society
2011
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3164170/ https://ncbi.nlm.nih.gov/pubmed/21889452 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2011.07.007 |
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