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Orientation dependence in fluorescent energy transfer between Cy3 and Cy5 terminally attached to double-stranded nucleic acids

We have found that the efficiency of fluorescence resonance energy transfer between Cy3 and Cy5 terminally attached to the 5′ ends of a DNA duplex is significantly affected by the relative orientation of the two fluorophores. The cyanine fluorophores are predominantly stacked on the ends of the heli...

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Hlavní autoři: Iqbal, Asif, Arslan, Sinan, Okumus, Burak, Wilson, Timothy J., Giraud, Gerard, Norman, David G., Ha, Taekjip, Lilley, David M. J.
Médium: Artigo
Jazyk:Inglês
Vydáno: National Academy of Sciences 2008
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC2516210/
https://ncbi.nlm.nih.gov/pubmed/18676615
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0801707105
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