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Sequence-Dependent Fluorescence of Cy3- and Cy5-Labeled Double-Stranded DNA

[Image: see text] The fluorescent intensity of Cy3 and Cy5 dyes is strongly dependent on the nucleobase sequence of the labeled oligonucleotides. Sequence-dependent fluorescence may significantly influence the data obtained from many common experimental methods based on fluorescence detection of nuc...

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Detalhes bibliográficos
Publicado no:Bioconjug Chem
Main Authors: Kretschy, Nicole, Sack, Matej, Somoza, Mark M.
Formato: Artigo
Idioma:Inglês
Publicado em: American Chemical Society 2016
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4796863/
https://ncbi.nlm.nih.gov/pubmed/26895222
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acs.bioconjchem.6b00053
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