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Orientation of Cyanine Fluorophores Terminally Attached to DNA via Long, Flexible Tethers

Cyanine fluorophores are commonly used in single-molecule FRET experiments with nucleic acids. We have previously shown that indocarbocyanine fluorophores attached to the 5′-termini of DNA and RNA via three-carbon atom linkers stack on the ends of the helix, orienting their transition moments. We no...

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Bibliografski detalji
Glavni autori: Ouellet, Jonathan, Schorr, Stephanie, Iqbal, Asif, Wilson, Timothy J., Lilley, David M.J.
Format: Artigo
Jezik:Inglês
Izdano: The Biophysical Society 2011
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC3164170/
https://ncbi.nlm.nih.gov/pubmed/21889452
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2011.07.007
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