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Specific labelling of the active site of T7 RNA polymerase.
We describe a method for specifically labelling T7 RNA polymerase at (or near) the active site. Enzyme molecules that have been modified by covalent attachment of a benzaldehyde nucleotide derivative in the presence of template DNA are subsequently incubated with radioactively labelled nucleoside tr...
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| Veröffentlicht in: | Nucleic Acids Res |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
Oxford University Press
1987
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| Online Zugang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC306404/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3684573/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/15.21.8773 |
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