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Specific labelling of the active site of T7 RNA polymerase.
We describe a method for specifically labelling T7 RNA polymerase at (or near) the active site. Enzyme molecules that have been modified by covalent attachment of a benzaldehyde nucleotide derivative in the presence of template DNA are subsequently incubated with radioactively labelled nucleoside tr...
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| Autors principals: | , , , |
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| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
1987
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| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC306404/ https://ncbi.nlm.nih.gov/pubmed/3684573 |
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