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Specific labelling of the active site of T7 RNA polymerase.

We describe a method for specifically labelling T7 RNA polymerase at (or near) the active site. Enzyme molecules that have been modified by covalent attachment of a benzaldehyde nucleotide derivative in the presence of template DNA are subsequently incubated with radioactively labelled nucleoside tr...

詳細記述

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書誌詳細
主要な著者: Schäffner, A R, Jorgensen, E D, McAllister, W T, Hartmann, G R
フォーマット: Artigo
言語:Inglês
出版事項: 1987
オンライン・アクセス:https://ncbi.nlm.nih.gov/pmc/articles/PMC306404/
https://ncbi.nlm.nih.gov/pubmed/3684573
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