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Specific labelling of the active site of T7 RNA polymerase.
We describe a method for specifically labelling T7 RNA polymerase at (or near) the active site. Enzyme molecules that have been modified by covalent attachment of a benzaldehyde nucleotide derivative in the presence of template DNA are subsequently incubated with radioactively labelled nucleoside tr...
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| 主要な著者: | , , , |
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| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
1987
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| オンライン・アクセス: | https://ncbi.nlm.nih.gov/pmc/articles/PMC306404/ https://ncbi.nlm.nih.gov/pubmed/3684573 |
| タグ: |
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