Specific labelling of the active site of T7 RNA polymerase.

We describe a method for specifically labelling T7 RNA polymerase at (or near) the active site. Enzyme molecules that have been modified by covalent attachment of a benzaldehyde nucleotide derivative in the presence of template DNA are subsequently incubated with radioactively labelled nucleoside tr...

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Udgivet i:Nucleic Acids Res
Main Authors: Schäffner, A R, Jorgensen, E D, McAllister, W T, Hartmann, G R
Format: Artigo
Sprog:Inglês
Udgivet: Oxford University Press 1987
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC306404/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3684573/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/15.21.8773
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