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Nucleotide-dependent conformational change governs specificity and analog discrimination by HIV reverse transcriptase

Single turnover studies on HIV reverse transcriptase suggest that nucleoside analogs bind more tightly to the enzyme than normal substrates, contrary to rational structural predictions. Here we resolve these controversies by monitoring the kinetics of nucleotide-induced changes in enzyme structure....

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Detalhes bibliográficos
Main Authors: Kellinger, Matthew W., Johnson, Kenneth A.
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 2010
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2867896/
https://ncbi.nlm.nih.gov/pubmed/20385846
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0913946107
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