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Using iTRAQ(®) Combined with Tandem Affinity Purification to Enhance Low-abundance Proteins Associated with Somatically-mutated EGFR Core Complexes in Lung Cancer
In this study we report a novel use for the iTRAQ(®) reagent combined with a peptide mass inclusion list to enhance the signal of low-abundance proteins during analysis by mass spectrometry. C-tagged-SH-EGFR was retrovirally-transduced into two mutant lung cancer cell lines (HCC827 and PC9) and the...
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| Main Authors: | , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
2010
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3017669/ https://ncbi.nlm.nih.gov/pubmed/20945942 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/pr100863f |
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