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Using iTRAQ(®) Combined with Tandem Affinity Purification to Enhance Low-abundance Proteins Associated with Somatically-mutated EGFR Core Complexes in Lung Cancer

In this study we report a novel use for the iTRAQ(®) reagent combined with a peptide mass inclusion list to enhance the signal of low-abundance proteins during analysis by mass spectrometry. C-tagged-SH-EGFR was retrovirally-transduced into two mutant lung cancer cell lines (HCC827 and PC9) and the...

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Gorde:
Xehetasun bibliografikoak
Egile Nagusiak: Haura, Eric B., Müller, André, Brietwieser, Florian P., Li, Jiannong, Grebien, Florian, Colinge, Jacques, Bennett, Keiryn L.
Formatua: Artigo
Hizkuntza:Inglês
Argitaratua: 2010
Gaiak:
Sarrera elektronikoa:https://ncbi.nlm.nih.gov/pmc/articles/PMC3017669/
https://ncbi.nlm.nih.gov/pubmed/20945942
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/pr100863f
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