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Tandem Affinity Purification in Drosophila Heads and Ovaries
Tandem affinity purification (TAP) (Pugi et al., 2001; Rigaut et al., 1999) is a method that uses a tagging approach of a target protein of interest for a two-step purification scheme in order to pull down protein complexes under native conditions and expression levels. The TAP tag consists of three...
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| Veröffentlicht in: | Bio Protoc |
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| Hauptverfasser: | , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
2012
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4814099/ https://ncbi.nlm.nih.gov/pubmed/27042686 |
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