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Optimisation of Downscaled Tandem Affinity Purifications to Identify Core Protein Complexes
In this study we show that via stable, retroviral-expression of tagged EGFR del (L747-S752 deletion mutant) in the PC9 lung cancer cell line and stable doxycycline-inducible expression of tagged Grb2 using a Flp-mediated recombination HEK293 cell system, the SH-TAP can be downscaled to 5 to 12.5 mg...
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| Main Authors: | , , , , , , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
2012
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3785125/ https://ncbi.nlm.nih.gov/pubmed/24077984 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.5584/jiomics.v2i1.81 |
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