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Isolation of Proteinase K-Sensitive Prions Using Pronase E and Phosphotungstic Acid
Disease-related prion protein, PrP(Sc), is classically distinguished from its normal cellular precursor, PrP(C), by its detergent insolubility and partial resistance to proteolysis. Molecular diagnosis of prion disease typically relies upon detection of protease-resistant fragments of PrP(Sc) using...
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| Main Authors: | , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Public Library of Science
2010
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3004958/ https://ncbi.nlm.nih.gov/pubmed/21187933 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0015679 |
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