By-passing in vitro screening—next generation sequencing technologies applied to antibody display and in silico candidate selection

In recent years, unprecedented DNA sequencing capacity provided by next generation sequencing (NGS) has revolutionized genomic research. Combining the Illumina sequencing platform and a scFv library designed to confine diversity to both CDR3, >1.9 × 10(7) sequences have been generated. This appro...

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Main Authors: Ravn, U., Gueneau, F., Baerlocher, L., Osteras, M., Desmurs, M., Malinge, P., Magistrelli, G., Farinelli, L., Kosco-Vilbois, M.H., Fischer, N.
格式: Artigo
語言:Inglês
出版: Oxford University Press 2010
主題:
Methods Online
在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC2995085/
https://ncbi.nlm.nih.gov/pubmed/20846958
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkq789
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