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The pyruvate kinase model system, a cautionary tale for the use of osmolyte perturbations to support conformational equilibria in allostery

In the study of rabbit muscle pyruvate kinase (M(1)-PYK), proline has previously been used as an osmolyte in an attempt to determine a role for preexisting conformational equilibria in allosteric regulation. In this context, osmolytes are small molecules assumed to have no direct interaction with th...

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Hlavní autoři: Fenton, Aron W, Johnson, Troy A, Holyoak, Todd
Médium: Artigo
Jazyk:Inglês
Vydáno: Wiley Subscription Services, Inc., A Wiley Company 2010
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC2975142/
https://ncbi.nlm.nih.gov/pubmed/20629175
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/pro.450
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