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Repeated probing of Southwestern blots using alkaline phosphatase stripping
Southwestern blotting is when a DNA sequence is used to probe DNA-binding proteins on an electrophoretic gel blot. It would be highly desirable to be able to probe a blot repeatedly with different DNA sequences. Alkaline phosphatase can remove 5′-phosphoryl groups from DNA and radiolabeled 5′-(32)P-...
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| Hlavní autoři: | , , |
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| Médium: | Artigo |
| Jazyk: | Inglês |
| Vydáno: |
2010
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| Témata: | |
| On-line přístup: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2964665/ https://ncbi.nlm.nih.gov/pubmed/20926088 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.chroma.2010.09.033 |
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