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Detection of mRNA by in situ hybridisation and in northern blot analysis using oligodeoxynucleotide probes labelled with alkaline phosphatase.

AIMS: To assess whether a reduction in intensity of signal observed using an alkaline phosphatase labelled oligodeoxynucleotide probe could be explained on the basis of procedural steps rather than reduced sensitivity. METHOD: Signal intensity was assessed on in situ hybridisation for pro-opiomelano...

Ausführliche Beschreibung

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Bibliografische Detailangaben
Veröffentlicht in:J Clin Pathol
Hauptverfasser: Farquharson, M. A., Harvie, R., Kennedy, A., McNicol, A. M.
Format: Artigo
Sprache:Inglês
Veröffentlicht: BMJ Publishing Group 1992
Schlagworte:
Online-Zugang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC495032/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1360478/
https://ncbi.nlm.nih.govhttps://doi.org/10.1136/jcp.45.11.999
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