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Preparation of oligodeoxynucleotide-alkaline phosphatase conjugates and their use as hybridization probes.
Short synthetic oligonucleotides have been covalently cross-linked to alkaline phosphatase using the homobifunctional reagent disuccinimidyl suberate. The oligomers, twenty-one to twenty-six bases in length, are complementary to unique sequences found in herpes simplex virus, hepatitis B virus, Camp...
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| Main Authors: | , , , |
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| Format: | Artigo |
| Language: | Inglês |
| Published: |
1986
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| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC311625/ https://ncbi.nlm.nih.gov/pubmed/3748805 |
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