Preparation of oligodeoxynucleotide-alkaline phosphatase conjugates and their use as hybridization probes.
Short synthetic oligonucleotides have been covalently cross-linked to alkaline phosphatase using the homobifunctional reagent disuccinimidyl suberate. The oligomers, twenty-one to twenty-six bases in length, are complementary to unique sequences found in herpes simplex virus, hepatitis B virus, Camp...
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| Publicat a: | Nucleic Acids Res |
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| Autors principals: | , , , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
Oxford University Press
1986
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| Accés en línia: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC311625/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3748805/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/14.15.6115 |
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