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Transcription Factor Proteomics: Identification by a Novel Gel Mobility Shift-Three-Dimensional Electrophoresis Method Coupled with Southwestern Blot and HPLC-Electrospray-Mass Spectrometry Analysis
Transcription factor (TF) purification and identification is an important step in elucidating gene regulatory mechanisms. In this study, we present two new electrophoretic mobility shift assay (EMSA)-based multi-dimensional electrophoresis approaches to isolate and characterize TFs, using detection...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2011
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3174475/ https://ncbi.nlm.nih.gov/pubmed/21880322 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.chroma.2011.08.023 |
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