Rapid generation of incremental truncation libraries for protein engineering using α-phosphothioate nucleotides

Incremental truncation for the creation of hybrid enzymes (ITCHY) is a novel tool for the generation of combinatorial libraries of hybrid proteins independent of DNA sequence homology. We herein report a fundamentally different methodology for creating incremental truncation libraries using nucleoti...

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Detalhes bibliográficos

Publicado no:	Nucleic Acids Res
Principais autores:	Lutz, Stefan, Ostermeier, Marc, Benkovic, Stephen J.
Formato:	Artigo
Idioma:	Inglês
Publicado em:	Oxford University Press 2001
Assuntos:	NAR Methods Online
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC29623/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11160936/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/29.4.e16
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