Rapid generation of incremental truncation libraries for protein engineering using α-phosphothioate nucleotides

Incremental truncation for the creation of hybrid enzymes (ITCHY) is a novel tool for the generation of combinatorial libraries of hybrid proteins independent of DNA sequence homology. We herein report a fundamentally different methodology for creating incremental truncation libraries using nucleoti...

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Bibliographic Details
Main Authors: Lutz, Stefan, Ostermeier, Marc, Benkovic, Stephen J.
Format: Artigo
Language:Inglês
Published: Oxford University Press 2001
Subjects:
NAR Methods Online
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC29623/
https://ncbi.nlm.nih.gov/pubmed/11160936
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