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Creating multiple-crossover DNA libraries independent of sequence identity
We have developed, experimentally implemented, and modeled in silico a methodology named SCRATCHY that enables the combinatorial engineering of target proteins, independent of sequence identity. The approach combines two methods for recombining genes: incremental truncation for the creation of hybri...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
The National Academy of Sciences
2001
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC58715/ https://ncbi.nlm.nih.gov/pubmed/11562494 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.201413698 |
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