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Creating multiple-crossover DNA libraries independent of sequence identity

We have developed, experimentally implemented, and modeled in silico a methodology named SCRATCHY that enables the combinatorial engineering of target proteins, independent of sequence identity. The approach combines two methods for recombining genes: incremental truncation for the creation of hybri...

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Bibliografiske detaljer
Udgivet i:Proc Natl Acad Sci U S A
Principais autores: Lutz, Stefan, Ostermeier, Marc, Moore, Gregory L., Maranas, Costas D., Benkovic, Stephen J.
Format: Artigo
Sprog:Inglês
Udgivet: National Academy of Sciences 2001
Fag:
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC58715/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11562494/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.201413698
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