Creating multiple-crossover DNA libraries independent of sequence identity
We have developed, experimentally implemented, and modeled in silico a methodology named SCRATCHY that enables the combinatorial engineering of target proteins, independent of sequence identity. The approach combines two methods for recombining genes: incremental truncation for the creation of hybri...
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| Udgivet i: | Proc Natl Acad Sci U S A |
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| Principais autores: | , , , , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
National Academy of Sciences
2001
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC58715/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11562494/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.201413698 |
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