Creating multiple-crossover DNA libraries independent of sequence identity

We have developed, experimentally implemented, and modeled in silico a methodology named SCRATCHY that enables the combinatorial engineering of target proteins, independent of sequence identity. The approach combines two methods for recombining genes: incremental truncation for the creation of hybri...

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Detalhes bibliográficos

Publicado no:	Proc Natl Acad Sci U S A
Principais autores:	Lutz, Stefan, Ostermeier, Marc, Moore, Gregory L., Maranas, Costas D., Benkovic, Stephen J.
Formato:	Artigo
Idioma:	Inglês
Publicado em:	National Academy of Sciences 2001
Assuntos:	Biological Sciences
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC58715/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11562494/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.201413698
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