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Directed evolution of a fucosidase from a galactosidase by DNA shuffling and screening

An efficient β-fucosidase was evolved by DNA shuffling from the Escherichia coli lacZ β-galactosidase. Seven rounds of DNA shuffling and colony screening on chromogenic fucose substrates were performed, using 10,000 colonies per round. Compared with native β-galactosidase, the evolved enzyme purifie...

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Библиографические подробности
Главные авторы: Zhang, Ji-Hu, Dawes, Glenn, Stemmer, Willem P. C.
Формат: Artigo
Язык:Inglês
Опубликовано: The National Academy of Sciences of the USA 1997
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Online-ссылка:https://ncbi.nlm.nih.gov/pmc/articles/PMC20752/
https://ncbi.nlm.nih.gov/pubmed/9114019
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