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Directed evolution of a fucosidase from a galactosidase by DNA shuffling and screening
An efficient β-fucosidase was evolved by DNA shuffling from the Escherichia coli lacZ β-galactosidase. Seven rounds of DNA shuffling and colony screening on chromogenic fucose substrates were performed, using 10,000 colonies per round. Compared with native β-galactosidase, the evolved enzyme purifie...
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| Hlavní autoři: | , , |
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| Médium: | Artigo |
| Jazyk: | Inglês |
| Vydáno: |
The National Academy of Sciences of the USA
1997
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| Témata: | |
| On-line přístup: | https://ncbi.nlm.nih.gov/pmc/articles/PMC20752/ https://ncbi.nlm.nih.gov/pubmed/9114019 |
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