Creating multiple-crossover DNA libraries independent of sequence identity

We have developed, experimentally implemented, and modeled in silico a methodology named SCRATCHY that enables the combinatorial engineering of target proteins, independent of sequence identity. The approach combines two methods for recombining genes: incremental truncation for the creation of hybri...

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Detalles Bibliográficos
Main Authors: Lutz, Stefan, Ostermeier, Marc, Moore, Gregory L., Maranas, Costas D., Benkovic, Stephen J.
Formato: Artigo
Idioma:Inglês
Publicado: The National Academy of Sciences 2001
Assuntos:
Biological Sciences
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC58715/
https://ncbi.nlm.nih.gov/pubmed/11562494
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.201413698
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